Phusion DNA Polymerases




概述


自從2003年問世以來,Thermo Scientific Phusion高保真DNA聚合酶建立了高保真PCR的新標準。Phusion DNA 高保真DNA聚合酶也逐漸成為更多PCR用戶的第一選擇,尤其是對這些基因組功能研究的客戶來說,必不可少。


35彩票会员登录 www.qbbsy.icu Phusion 高保真DNA聚合酶,是一種具有校對功能的DNA聚合酶,來源于類熱球菌(Pyrococcus-like),融合有DNA結合域。借助這種融合技術,Phusion DNA聚合酶具有一般單酶無法實現的PCR擴增的速度和準確率,即使是擴增復雜模板也是效率很高。除此之外,Phusion DNA聚合酶對多種抑制劑具有很強的耐受性,只需要極少的優化步驟就可以實現PCR的有效擴增,對于熱啟動PCR有需求的客戶來說,Phusion Hot Start II DNA Polymerase是您的理想選擇。

Phusion高保真DNA聚合酶宣傳冊

產品特點


● 精確-是目前保真度最高的熱穩定性聚合酶(是Taq的50倍, Pfu的6倍)


● 強勁-更高的成功率和更少的優化步驟


● 快速-延伸速率為15-30s/kb


● 高產-低酶量、高產量


● 特異-獨特的熱啟動修飾,實現“零時間”激活,減少了非特異性擴增和引物降解


產品應用

高保真PCR、快速PCR、熱啟動PCR、長片段擴增、高通量PCR

PCR擴增的高保真性


在很多應用中,比如,定點突變或者從DNA到蛋白的翻譯過程中,維持DNA序列的準確性在PCR擴增過程中是很關鍵的。一個錯誤的核酸摻入將會改變編碼序列從而改變翻譯后的蛋白質氨基酸序列,這樣將影響蛋白的折疊及功能特性。另一方面,一個氨基酸的缺失將會破壞整個正確的讀碼框。

Phusion DNA 聚合酶具有極低的錯賠率,從而建立了Taq? PCR反應的金標準,用基于修飾的lacI方法檢測結果顯示Phusion DNA 聚合酶具有50倍以上Taq DNA聚合酶及大于6倍的Pfu?DNA的保真度。(see Figure 1).

bar graph showing relative fidelity values of different DNA polymerases

Figure 1. Relative fidelity values of different DNA polymerases. Fidelity = 1 / error rate.?The low error rate of Phusion DNA Polymerase was recently confirmed by studies using 454 sequencing2 and Illumina sequencing3. With 454 sequencing, Phusion DNA Polymerase was shown to have the lowest error rate compared to other DNA polymerases (see Table 1).

Table 1.?Error rates determined by 454 sequencing for seven different DNA polymerases after PCR amplification of four different exons from the human TP53 oncogene, using a clonal TP53 plasmid as starting template.



a Error rate, number of errors (miscalled bases, inserted or deleted bases) divided by total number of bases.bDot, three successive negative flows during 454 sequencing. Table reprinted with the permission from the corresponding author and the journal editor.
B.F. Frey & B. Suppmann (1995) Demonstration of the Expand PCR System's greater fidelity and higher yields with a lacI-based fidelity assay. Biochemica 2, 34-35.Vandenbroucke et al. (2011) Minor variant detection in amplicons using 454 massive parallel pyrosequencing: experiences and considerations for successful applications. Biotechniques. 53:167-177.Kinde et al. (2011) Detection and quantification of rare mutations with massively parallel sequencing. PNAS. 108(23):9530–9535.


產品信息:


貨號

產品名稱

規格

目錄價¥

Phusion高保真PCR

F-530S

Phusion High-Fidelity DNA Polymerase

100 U(2U/ul)

1027

F-530L

Phusion High-Fidelity DNA Polymerase

500 U(2U/ul)

4077

F-534S

Phusion Green High-Fidelity DNA Polymerase

100 Unit(2U/ul)

1027

F-534L

Phusion Green High-Fidelity DNA Polymerase

500 Unit(2U/ul)

4077

F-553S

Phusion High-Fidelity PCR Kit

50 x 50 μL rxns

644

F-553L

Phusion High-Fidelity PCR Kit

200 x 50 μL rxns

2178

F-532S

Phusion High-Fidelity PCR Master Mix with GC Buffer

100 x 50 μL rxns

1657

F-532L

Phusion High-Fidelity PCR Master Mix with GC Buffer

500 x 50 μL rxns

6616

F-531S

Phusion High-Fidelity PCR Master Mix with HF Buffer

100 x 50 μL rxns

1657

F-531L

Phusion High-Fidelity PCR Master Mix with HF Buffer

500 x 50 μL rxns

6616

Phusion高保真快速PCR Mix

F-548S

Phusion Flash High-Fidelity PCR Master Mix

100 rxns

772

F-548L

Phusion Flash High-Fidelity PCR Master Mix

500 rxns

3094

Phusion 高保真熱啟動DNA聚合酶及Mix

F-549S

Phusion Hot Start II High-Fidelity DNA Polymerase

100 U(2U/ul)

1245

F-549L

Phusion Hot Start II High-Fidelity DNA Polymerase

500 U(2U/ul)

4951

F-537S

Phusion Green HSII HF DNA pol

100 Unit(2U/ul)

1245

F-537L

Phusion Green HSII HF DNA pol

500 Unit(2U/ul)

4951

F-565S

Phusion Hot Start II High-Fidelity PCR master Mix

100 x 50 μL rxns

1715

F-565L

Phusion Hot Start II High-Fidelity PCR master Mix

500 x 50 μL rxns

6834

F-566S

Phusion Green Hot Start II High-Fidelity PCR master Mix

100 x 50 μL rxns

1715

F-566L

Phusion Green Hot Start II High-Fidelity PCR master Mix

500 x 50 μL rxns

6834

Phusion高保真定點突變試劑盒

F-541

Phusion Site-Directed Mutagenesis Kit

20 rxns

1455

Phusion U高保真酶及Mix

F-556S

Phusion U Green HS Polymerase

100 Unit(2U/ul)

1397

F-556L

Phusion U Green HS Polymerase

500 Unit(2U/ul)

5523

F-564S

Phusion U Green Multiplex PCR Master Mix

100 x 50 μL rxns

1959

F-564L

Phusion U Green Multiplex PCR Master Mix

500 x 50 μL rxns

7784

F-555S

Phusion U HS DNA Polymerase

100 Unit(2U/ul)

1397

F-555L

Phusion U HS DNA Polymerase

500 Unit(2U/ul)

5523

F-533S

Phusion U HS PCR Master Mix

100 x 50 μL

1985

F-533L

Phusion U HS PCR Master Mix

500 x 50 μL

7893

F-562S

Phusion U Multiplex PCR Master Mix

100 x 50 μL rxns

1959

F-562L

Phusion U Multiplex PCR Master Mix

500 x 50 μL rxns

7784

Phusion高保真DNA聚合酶Buffer

F-519L

Phusion GC Buffer Pack

4 x 1.5 mL

152

F-521L

Phusion GC Buffer Pack, Detergent-Free

4 x 1.5 mL

152

F-518L

Phusion HF Buffer Pack

4 x 1.5 mL

152

F-520L

Phusion HF Buffer Pack, Detergent-Free

4 x 1.5 mL

152

F-539L

Phusion Green GC Buffer Pack

4 x 1.5 mL

152

F-538L

Phusion Green HF Buffer Pack

4 x 1.5 mL

152

 

 

 

 

 

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